Method of extracting and modifying proteins or albuminous matter.



the fermentation of malt or other CHARLES C. RINGLER AND JOE-TelBEERHALTER, (31 EU 03: THIRTY-THREE AND ONE-THIRD ONE-HUNDRED PANEL 01EDULUTH, LlIIINNESO'I'A, A CORPORATION O;

METHQD 01 EXERACTING AND MODIFYING PFQUTEIH ALI lilo Drawing.

To an whom 455 may concern."

Be it known that we, CHARLES C- RINGLER and JOHN BEERHALTER, citizens ofthe United States, and residents of Duluth, in the county ofSt. Louisand State of Minnesota, have invented a new and Improved Method ofExtracting and Modifying Pro-' teins or Albuminous Matter, of which thefollowing is a full, clear, and exact description.

Our invention relates to a method of extracting and modifying protein oralbuminous' matter from substances containing it, and is particularly,adaptable to the extraction and modification of protein or albuminousmatter from yeast cells.

Before proceeding to a more detailed description of our invention itmust be clearly understood that although the description of the methodis specific as to the extraction and modification of protein frombrewers yeast, the method is by no means limited to this particularsubstance and can be as efficiently utilized with any substancecontaining protein or albuminous matter. By modification is meant thechemical reaction of ammonia on the albuminous constituents, whichprevents coagulation and keeps them in solution.

Yeasts grown by fermentation are gener ally contaminated with substancesof the extract fermented that may give a disagreeable taste to theyeast. This contaminating substance can be dissolved chemically orremoved mechanically. Yeast resulting from brewing extracts which havebeen hopped during the brewing operation is contaminated with hop resin.lhe resins are preferably eliminated by the addition of alkalis ormechani cally by forcing theyeast through a suitable filtering surfacewhich Will retain the resins.

The yeast treated as above is digested with a solution of ammonia(ammonium hydrate), preferably under pressure and While subjected toheat; or in lieu of pressure and heat steam may be used. The pressureand heat help to Weaken the cellular structure of the yeast andfacilitate the action of the ammonia in solution on the contents of thecells, which are protein or albuminous matter. The cellulose matter ofSpecification of Letters Patent.

.Jlppllcation filed March 13, 1918.

tein. It may be Serial Iii-i.

the yeast preci tion and the liquid ma;

The treatment of th under pressure and exclusion of of ammonia is reuior. sure in the digester or evaporator. The tein or albumiiious sub ablein a solution ricliing beveragesv 2 user adulterated, or adult anysuitable substan tractive or palatable.

We claim:

15A method of e ing protein or al umw yeast, which consists i With asolution of ZUYW" cess of ammonia by eve rating the solution fro! 2. Amethod of at protein from yeast, Wil ing the yeast under p of ammonia todissolve in the yeast, then re ammonia by evapor" the resulting solution3 3. A method of extra protein or albuminous which consists in digsjected to pressure and monia solution to dissol tained in the yeast,rem-z ammonia, and separatinv lution from the sedimei' 4. A method ofextract protein or albuminous n yeast, which consists eln the yeast thesubstances co tam digesting the so treated yeast monia solution, Whilesubjected to and heat, to dissolve the protei: in the yeast, rem vingthe monia, and separating re from the sedimen 5. A method of extractingprotein or albuminous ma yeast, which consists in elinnresins from theyeast,

- protein or a'lhuminous matter from brewers,

treated yeast with an ammonia 'solutioi1,, v while subjected to pressureand heat, to dissolve the protein'contained in the yeast, and

separating the resulting solution from the sediment. '6. A method ofextracting and modifying yeast, which consists in treating the yeastwithalkalis to dissolve the hop resins con- ,tained'in the yeast,digestingthe so-treatedyeast with an ammonia solution while sub-' jectedto pressure and heat, and separating the'resulting solution from thesediment;

In testimony whereof we have signed our names to this specification inthe presence of 15 two subscribing witnesses.

